Temperature Effects on Microbial Communities of Sea Fan Coral as an Indicator of Disease Susceptibility

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Temperature Effects on Microbial Communities of Sea Fan Coral as an Indicator of Disease Susceptibility

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dc.contributor.advisor Kim, Kiho en_US
dc.contributor.author Timme, Walker en_US
dc.contributor.other Carlini, David en_US
dc.contributor.other DeCicco-Skinner, Kathleen en_US
dc.date.accessioned 2012-08-22T15:01:36Z
dc.date.available 2012-08-22T15:01:36Z
dc.date.created 2011 en_US
dc.date.issued 2012-08-22
dc.identifier.other Timme_american_0008N_10143 en_US
dc.identifier.uri http://hdl.handle.net/1961/11128
dc.description Degree awarded: M.S. Biology. American University en_US
dc.description.abstract Coral reefs are in decline due to increases in coral disease. We suspect that one of the main sources of coral immunity is the strength the residential, native microbiota. Based on vertebrate models I suspect that disturbing the normal microbial community will lead to disease. The goal of this study is to use the coral-microbiota-disease hypothesis as a tool to establish how an increase in sea surface temperature leads to disease. I tested this by first exposing cuttings of sea fan coral (<italic>Gorgonia ventalina</italic>) to average summer sea surface temperature as well as to higher than average temperatures. Then, I analyzed the microbial community structure using denaturing gradient gel electrophoresis (DGGE). Finally, I identified individual bands through sequencing. I found that there were not significant differences in the microbial communities due to changes in temperature. However, the microbial community structures varied greatly when comparing coral on reefs and those in aquaria. en_US
dc.format.extent 56 p. en_US
dc.format.mimetype application/pdf en_US
dc.publisher American University en_US
dc.subject Environmental science en_US
dc.subject Microbiology en_US
dc.subject Biology en_US
dc.subject.other coral en_US
dc.subject.other DGGE en_US
dc.subject.other disease en_US
dc.subject.other Gorgonia en_US
dc.subject.other microbiota en_US
dc.subject.other temperature en_US
dc.title Temperature Effects on Microbial Communities of Sea Fan Coral as an Indicator of Disease Susceptibility en_US
dc.type Text en_US
dc.type Dissertation en_US


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